Synchronization of mouse islets of Langerhans by glucose waveforms
نویسندگان
چکیده
منابع مشابه
Synchronization of mouse islets of Langerhans by glucose waveforms.
Pancreatic islets secrete insulin in a pulsatile manner, and the individual islets are synchronized, producing in vivo oscillations. In this report, the ability of imposed glucose waveforms to synchronize a population of islets was investigated. A microfluidic system was used to deliver glucose waveforms to ∼20 islets while fura 2 fluorescence was imaged. All islets were entrained to a sinusoid...
متن کاملMONOPHASIC STATE OF INSULIN SECRETION IN ISOLATED RAT ISLETS OF LANGERHANS BY K+, Ca2+ AND GLUCOSE
Isolation of rat islets of Langerhans was carried out by using collagenase and the destruction of exocrine tissue was performed through the injection of Hanks' solution into the common bile duct. The identification of the islets was done by techniques including the use of a stereomicroscope against a black background. Vital staining was done by an injection of neutral red solution through t...
متن کاملMicrofluidic system for generation of sinusoidal glucose waveforms for entrainment of islets of Langerhans.
A microfluidic system was developed to produce sinusoidal waveforms of glucose to entrain oscillations of intracellular [Ca(2+)] in islets of Langerhans. The work described is an improvement to a previously reported device where two pneumatic pumps delivered pulses of glucose and buffer to a mixing channel. The mixing channel acted as a low pass filter to attenuate these pulses to produce the d...
متن کاملmonophasic state of insulin secretion in isolated rat islets of langerhans by k+, ca2+ and glucose
isolation of rat islets of langerhans was carried out by using collagenase and the destruction of exocrine tissue was performed through the injection of hanks' solution into the common bile duct. the identification of the islets was done by techniques including the use of a stereomicroscope against a black background. vital staining was done by an injection of neutral red solution through ...
متن کاملThe dual function of glucose in islets of Langerhans.
It is widely accepted that metabolites of glucose rather than glucose itself trigger insulin release from p-cells. If so, level changes of such metabolites ought to occur in P-cells within seconds of a glucose pulse. To examine this hypothesis, glucose, glucose-6-P, fructose-diP plus triose-P, 6-P-gluconate, ATP, and P-creatine were measured in individual rat islets after intravenous infusion f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: American Journal of Physiology-Endocrinology and Metabolism
سال: 2011
ISSN: 0193-1849,1522-1555
DOI: 10.1152/ajpendo.00248.2011